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celltrace violet (ctv) fluorescent dye  (Thermo Fisher)


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    Thermo Fisher celltrace violet (ctv) fluorescent dye
    <t>CellTrace</t> Violet <t>(CTV)</t> reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.
    Celltrace Violet (Ctv) Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltrace violet (ctv) fluorescent dye/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    celltrace violet (ctv) fluorescent dye - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Trypanosoma cruzi amastigotes have a reduced replication rate during chronic stage infections"

    Article Title: Trypanosoma cruzi amastigotes have a reduced replication rate during chronic stage infections

    Journal: bioRxiv

    doi: 10.1101/2020.08.04.236240

    CellTrace Violet (CTV) reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.
    Figure Legend Snippet: CellTrace Violet (CTV) reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.

    Techniques Used: Infection, Incubation, Standard Deviation



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    celltrace violet (ctv) fluorescent dye  (Thermo Fisher)
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    Thermo Fisher celltrace violet (ctv) fluorescent dye
    <t>CellTrace</t> Violet <t>(CTV)</t> reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.
    Celltrace Violet (Ctv) Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltrace violet (ctv) fluorescent dye/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    celltrace violet (ctv) fluorescent dye - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    CellTrace Violet (CTV) reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.

    Journal: bioRxiv

    Article Title: Trypanosoma cruzi amastigotes have a reduced replication rate during chronic stage infections

    doi: 10.1101/2020.08.04.236240

    Figure Lengend Snippet: CellTrace Violet (CTV) reduces T. cruzi infectivity and inhibits intracellular proliferation. (A) T. cruzi CL-Luc::Scarlet trypomastigotes were incubated with either 5 or 10 µM CTV for 20 minutes and used to infect Vero cell monolayers at a multiplicity of infection of 10:1 (Materials and methods). 18 hours later, infection efficiency was determined by inspecting a total of 2,203 (control), 3,781 (5 µM), and 3,840 (10 µM) Vero cells. At least 300 of these were infected in each case. Each data point corresponds to a randomly acquired image and represents the mean percentage of cells infected. Differences between columns were analysed using a parametric one-way ANOVA with Tukey's post hoc pair wise comparisons. ****p=≤0.0001. (B) Vero cells infected with CTV−ve or CTV+ve trypomastigotes (as above) were incubated for the time periods indicated. The numbers of amastigotes per infected host cell were then determined by analysing >300 infected cells per treatment. Error bars represent the standard deviation from the mean. Data were analysed using a Wilcoxon rank sum test. (C) Images of Vero cells 36 hours after infection with CTV−ve or CTV+ve trypomastigotes. Red, fluorescent T. cruzi amastigotes. Fluorescent parasites containing the CTV tracer dye appear as purple on a red fluorescent background. Size bars=20 µM (D) Images of Vero cells 5 days after infection with trypomastigotes that had been incubated with various concentrations of CTV, as indicated. Blue, intracellular vesicles containing CVT. See also supplementary Video 1. Size bars=20 µM, except where indicated; *=50 µM.

    Article Snippet: T. cruzi trypomastigotes were isolated by centrifugation and allowed to recover for 2 hours at 37ºC in high-glucose DMEM medium with 10% FBS, and then labelled with the CellTrace Violet (CTV) fluorescent dye (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Infection, Incubation, Standard Deviation